real time workshop tool Search Results


genotyping  (Transnetyx)
99
Transnetyx genotyping
Genotyping, supplied by Transnetyx, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genotyping - by Bioz Stars, 2026-03
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real-time pcr (taqman) primer design  (GenScript corporation)
90
GenScript corporation real-time pcr (taqman) primer design
Real Time Pcr (Taqman) Primer Design, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/real-time pcr (taqman) primer design/product/GenScript corporation
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real-time pcr (taqman) primer design - by Bioz Stars, 2026-03
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real time pcr clustering tool version1.06.00  (TOHOKU CHEMICAL Co Ltd)
90
TOHOKU CHEMICAL Co Ltd real time pcr clustering tool version1.06.00
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Real Time Pcr Clustering Tool Version1.06.00, supplied by TOHOKU CHEMICAL Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/real time pcr clustering tool version1.06.00/product/TOHOKU CHEMICAL Co Ltd
Average 90 stars, based on 1 article reviews
real time pcr clustering tool version1.06.00 - by Bioz Stars, 2026-03
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population analysis tool  (Real Time Genomics)
90
Real Time Genomics population analysis tool
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Population Analysis Tool, supplied by Real Time Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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population analysis tool - by Bioz Stars, 2026-03
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real time genomics tool vcfeval  (Real Time Genomics)
90
Real Time Genomics real time genomics tool vcfeval
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Real Time Genomics Tool Vcfeval, supplied by Real Time Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tool real-time monitoring control thin film deposition processes  (Intelligent Sensor Technology Inc)
90
Intelligent Sensor Technology Inc tool real-time monitoring control thin film deposition processes
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Tool Real Time Monitoring Control Thin Film Deposition Processes, supplied by Intelligent Sensor Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tool real-time monitoring control thin film deposition processes/product/Intelligent Sensor Technology Inc
Average 90 stars, based on 1 article reviews
tool real-time monitoring control thin film deposition processes - by Bioz Stars, 2026-03
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real-time pcr primer and probe design tool  (PrimerDesign Inc)
90
PrimerDesign Inc real-time pcr primer and probe design tool
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Real Time Pcr Primer And Probe Design Tool, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/real-time pcr primer and probe design tool/product/PrimerDesign Inc
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real-time pcr primer and probe design tool - by Bioz Stars, 2026-03
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integrated analysis tool rtgfamily v.3.6.2  (Real Time Genomics)
90
Real Time Genomics integrated analysis tool rtgfamily v.3.6.2
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Integrated Analysis Tool Rtgfamily V.3.6.2, supplied by Real Time Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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integrated analysis tool rtgfamily v.3.6.2 - by Bioz Stars, 2026-03
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software real-time measurement tool  (Sonosite Inc)
90
Sonosite Inc software real-time measurement tool
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Software Real Time Measurement Tool, supplied by Sonosite Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lingene k real time pcr tool  (Bioer Technology Co Ltd)
90
Bioer Technology Co Ltd lingene k real time pcr tool
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Lingene K Real Time Pcr Tool, supplied by Bioer Technology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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real-time collaboration tool for biomolecular modeling  (BioCore Inc)
90
BioCore Inc real-time collaboration tool for biomolecular modeling
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Real Time Collaboration Tool For Biomolecular Modeling, supplied by BioCore Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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real-time spectral histogram analysis tool  (Tektronix inc)
90
Tektronix inc real-time spectral histogram analysis tool
(a) Real-time <t>PCR</t> analyses <t>of</t> <t>glycogenes</t> revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).
Real Time Spectral Histogram Analysis Tool, supplied by Tektronix inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(a) Real-time PCR analyses of glycogenes revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).

Journal: Scientific Reports

Article Title: A Cancer-specific Monoclonal Antibody Recognizes the Aberrantly Glycosylated Podoplanin

doi: 10.1038/srep05924

Figure Lengend Snippet: (a) Real-time PCR analyses of glycogenes revealed that LN229 cells were clustered into glioblastoma tissues (WHO Grade IV); by contrast, another glioblastoma cell line, LN319, was clustered into WHO Grade II/III. (b) Lectin microarray. Podoplanins on LN229/hPDPN, LN319, and CHO/hPDPN cells were solubilized using PBST. Then, 100 μl of purified podoplanin was applied to the lectin array. After incubation at 20°C for 17 h, the reaction solution was discarded. The glass slide was scanned using a GlycoStation Reader 1200. (c) Western-blot analyses. Purified podoplanin (0.1 μg) was boiled in SDS sample buffer, electrophoresed, and transferred onto a PVDF membrane. After blocking, the membrane was incubated with primary antibodies (NZ-1, 5D4) or biotinylated lectin (WGA, STL) and then with peroxidase-conjugated secondary antibodies or streptavidin-HRP; the membrane was developed with ECL-plus reagents using a Sayaca-Imager. L, LN229/hPDPN; C, CHO/hPDPN. (d) Schematic illustration of the glycan structure of podoplanin. Podoplanin in LN229/hPDPN cells possesses both polylactosamine and sialylated core 1, whereas podoplanin in CHO/hPDPN cells possesses only sialylated core 1. (e) Structure and synthesis of keratan sulfate. (f) Transcript levels for KSGal6ST, GlcNAc6ST-1, GlcNAc6ST-5, β3GnT7, and β4GalT4 genes in each cell line were measured using real-time PCR. Values normalized to the level of β-actin transcripts are presented. The error bars show the standard deviation of three independent experiments. Statistical analysis was performed using Student's t -test (* p < 0.05, ** p < 0.01).

Article Snippet: Clustering analysis against glycogenes was performed using the Real Time PCR Clustering Tool (version1.06.00; Research Institute of Bio-System Informatics, Tohoku Chemical Co,.

Techniques: Real-time Polymerase Chain Reaction, Microarray, Purification, Incubation, Western Blot, Membrane, Blocking Assay, Glycoproteomics, Standard Deviation